IL-3 ELISA Kit

Produktnummer ABIN5026937

Dieses Human IL-3 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-3 zu quantifizieren.

Kurzübersicht für IL-3 ELISA Kit (ABIN5026937)

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-3 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-3 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 9.4 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human IL-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL-3 (Interleukin 3)

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie